In last year's News Letter, I indicated that I would be sorting through the stocks I had stored at ISU. This task has been completed and there are now two sources of the stocks that were retained, which are now available for distribution to interested researchers. One set of stocks was sent to the Maize Genetic Cooperation Stock Center at the University of Illinois, Urbana, Il. The second set of stocks has been retained at ISU. The Coop stocks are those I thought would be of more general interest to the maize genetic community, while the ISU stocks are those that I thought would be of more interest to Mutator connoisseurs. Some item have been retained in both collections. Below are lists of the two sets of stocks. All stocks except those used as planting sources for, or produced in, the years 1989. 1990, and 1991 have been stored in our cold storage facilities. These facilities were not the most reliable and at times the stocks were in less than optional conditions for periods of a week or two until repairs could be made. Thus, it would be advisable to test a few kernels of each stock for germination before planting them in the field. The older the stocks the higher the likelihood of poor germination. DEFINITIONS OF TERMS AND SYMBOLS. Mutator (Mu) - A stock that has the potential for Mutator activity. MuDR - The autonomous element of the Mutator system. Mu2 - Stocks resulting from the crossing of two active Mutator lines, which have previously been propagated by outcrossing. Mu3 - Stocks resulting from the crossing of an active Mutator line with a Mu2 stock. Mu4 - Stocks resulting from the crossing of two Mu2 lines. Mu5 - Stocks resulting from the crossing of active Mutator lines as follows: 1 X 4 = Mu X Mu4 or 2 X 3 = Mu2 X Mu3). Similarly for Mu6, Mu8, etc. Outcrossed - Used when a Mutator stock or a Mutator-induced mutant is outcrossed one or more times to a Stand. or a Pl. Aleur. line or other genetic stocks. Gene symbol-Mu - A Mutator-induced allele or event at the locus the symbol represents. Gene symbol-Mum - A mutable Mutator-induced allele at the the locus the symbol represents. Gene symbol-Mum stable - A stable derivative of a Mum allele. Gene symbol-Mus - A Mutator-induced mutant that was non-mutable when first observed. (1:1) - When associated with a genotype, indicates that the particular Mutator-induced mutant segregated approximately 50% mutable to 50% stable mutant kernels. These stocks have the possibility of segregating for one or more MuDR elements. (3:1) - When associated with a genotype, indicates that the particular Mutator-induced mutant segregated approximately 75% mutable to 25% stable mutant kernels. These stocks have the possibility of segregating two or more MuDR elements. a1 sh2 segregant from (1:1) - One half of these stocks should carry one or more MuDR elements. "Tested negative for Mutator activity." - Samples of kernels from the ears of these stocks were sown and the resulting plants self-pollinated. None of the self-pollinated ears segregated for new mutants. "Tested positive for Mutator activity." - Samples of kernels from the ears of these stocks were sown and the resulting plants self-pollinated. Some of the self-pollinated ears segregated for new mutants. "Sibling plant(s) segregated for a new mutant" - Outcrossed ears from a family derived from the kernels of an active Mutator plant, which itself had been outcrossed. One or more of the sibling plants of that family segregated for a new mutant. (Note in these stocks and those in the previous definition have a high likelihood of having Mutator activity. However, some plants resulting from these crosses will have lost Mutator activity. Thus, to be sure an active plant has been selected for crossing it should be tested in some manner to determine that Mutator activity is retained. (See the paper in the News Letter entitled, "Some Thoughts on the nature and Utilization of the Mutator System".) Pl. Aleur - a purple aleurone stock homozygous for all alleles necessary for aleurone pigmentation. Pl. (plus a genotype) - The given genotype is in a purple aleurone background as defined above. Stand. - anyone of several different standard (F1 hybrid) lines. homo. - Homozygous. 2 - Mutability class with only one or two reversion spots. 2 X 2 = 1 - Results observe when two Class 2 stocks are crossed yielding progeny with only stable kernels (= Class 1). Hi loss - A stock of an intensely mutable Mutator-induced mutant, which upon outcrossing has lost mutability in one generation, (i.e., the stock has become stable). STOCKS SENT TO THE MAIZE GENETICS COOPERATION (Stocks listed below have been in cold storage) GENOTYPE COMMENTS YEAR BOX NO Sienna (necrotic seedling) 1973 1 a1 y10 (w-8624) 1973 1 R-Scm2/C-I TB-9sb 1973 1 w3-pas 8686 1973 1 cl1-7716 Clm2 1973 1 vp9-4889 (pastel) 1974 1 Compound TB-7Lb-4L 4698 1981 1 Compound TB-9Sb-4L 6504 1981 1 w*-8657 (Chromosome 6) 1981 1 Compound TB-1Sb-2Lc 1977 1 Compound TB-3La-2L 7285 1977 1 al-Brawn 1977 1 o5 1977 1 w3-8686 (pastel seedling) 1977 1 w3-8686 (pastel seedling) 1978 1 o5 1979 1 o7 1979 1 y1 wx1 gl1 sh1 1979-80 1 y1 wx1 gl8 1979-80 1 y1 wx1 gl1 1980 1 sh1-Mu 9026 1980 1 sh1-Mu 1746 1980 1 sh1-Mu 9626 1980 1 vp5-Mumm 1 1980 1 Compound TB-9Sb-4L 6222 1980 1 sh1-Mu 9026 1980-81 1 sh1-Mu 9626-31 1980-81 1 Inversion 3c 1978 1 Inversion 7S 1978 1 Inversion 7a 1978 2 sh1-Mu 9026 1980-81 2 sh1-Mu 9626 1980-81 2 Compound TB-3La-2S 6270 1980 Compound TB-1Sb-2Lc (Homozygous) 1977 2 Compound TB-1Sb-2L 4464 1980 2 Compound TB-3La-2L 7285 1977 2 Compound TB-1La-4L 4692 1981 2 Compound TB-9Sb-4L 6504 1981 2 Stand/(sh1-Mu 1746/+) 1982 2 a2-Mum3 1982 2 y1 wx1 bt2-Mu 9626 (bt2-Mu has a high 1982 reversion frequency.) a2-Mus (Independent isolates - 1, 2, 3.) 1981-82 2 a1-Mus (Independent isolates - 1,2.) 1982-83 2 a1-Mum (Independent isolates - 2, 3.) 1982-83 2 bz1-Mum (Independent isolates - 1, 2, 3, 4, 1982-83 5, 6, 7.) Six new putative Mu-induced opaques, all„ 1980 & 2 tested negative with o2. „ 1982 a1-Mus (Independent isolates - 1, 2, 3, 4.) 1982 2 sh1-Mu 4020 1982 2 sh1-Mu 1746 1982 2 y1 wx1 gl1 bt2 1981 2 sh1-Mu 9026 1981 2 c2/TB-1Sb-2L 4464-4Lf (Double compound TB) 1981 2 sh1-Mu 9026 (Homozygous) 1981 sh1-Mu 9626 (Homozygous) 1981 2 sh1-Mu 1746 (Homozygous) 1981 2 y1 wx1 gl8 1981 2 y1 wx1 gl1 bt2 1981 2 y1 wx1 bt2-Mu 9626 (bt2-Mu has a high 1981 reversion frequency.) a1-Mum1 (Self-pollinated) 1981-82 2 Pl. Aleur./a1-Mum1 1981-82 2 a1-Mum2 (Self-pollinated) 1981-82 2 a1 sh2/a1-Mum2 1981-82 2 Pl. Aeur./a1-Mum2 1981-82 2 a1-Mum3 (Self-pollinated) 1981-82 3 a1 sh2/a1-Mum3 1981-82 3 Pl. Aleur/a1-Mum3 1981-82 3 a1-Mus (Independent isolates - 1, 2, 3. - 1981-82 3 Self-pollinated) a1 sh2/a1-Mus3 1981-82 3 a1-Mu(s)4 1981-82 3 a1 sh2/a1-Mu(s)4 1981-82 3 al-Brawn/TB-3La-2S 6270 1982 3 Tb-3La-2S 6270/Stand. (TB tested positive.) 1982 3 (w3-Everett#1/w3) X TB-1Sb-2Lc 1982 3 TB-1Sb-2Lc/Stand. (TB tested positive.) 1982 3 w3-8686 (pastel)/TB-1Sb-2L 4464 1982 3 TB-ISb-2L 4464/Stand. (TB tested positive.) 1982 3 (w3-Everett#1/w3) X TB-3La-2L 7285 1982 3 TB-3La-2L 7285/Stand. (TB tested positive.) 1982 3 w3-8686 (pastel)/TB-3La-2L 7285 1982 3 c2/TB-1La-4L 4692 1982 3 TB-1La-4L 4692/W22 Rscm2 (TB tested positive.)1982 3 c2/TB-9Sb-4L 6504 1982 3 c2/TB-1Sb-2L 4464-4Lf (Double compound TB.) 1982 3 bz1-Mum (Independent isolates - 1, 2, 3, 1982 3 4, 17.) TB-7Lb-4L 4698/W22 R-scm2 1982 3 c2/TB-7b-4L 4698 1982 3 sh1-Mu 9026 1982 3 sh1-Mu 1746 1982 3 a1-Mus1/a1 sh2 (Self-pollinated.) 1982 3 a1-Mus2/a1 sh2 (Self-pollinated.) 1982 3 a1-Mus3/a1 sh2 (Self-pollinated.) 1982 3 a1-Mus4/a1 sh2 (Self-pollinated.) 1982 3 sh1-Mu 9626 1982 3 inv. 3c (In Kys inbred) 1982 3 inv. 3c (In Kys inbred) 1982 4 inv. 7a (In Kys inbred) 1982 3 a2-Mum1/a2 bt1 1982 4 a2-Mum2/a2 bt1 1982 4 a1-Mum1 1982 4 a1-Mum2 1982 4 a1-Mum3 1982 4 Rhoade's a1 mutable 1982 4 y1 wx1 gl1 bt2 1982-83 4 bz1-Mum (Independent isolates - 2, 3, 4.) 1982-83 4 a1-Mum (Independent isolates - 1, 2, 3.) 1982-83 5 Rhoade's bz1-m1 1983 5 y1 wx1 gl1 bt2-Mu 9626 1983 5 r/TB-1019 1983 5 brn1 1983 5 sh1-Mu 9552 1983 5 sh1-Mu 4020 1983 5 bt1-Mu 4206 1983 5 fl2-Mu 9234 1983 5 a1-mus (Independent isolates - 1, 2, 3, 4.) 1983 5 sh1-Mu 4955 1983 5 sh1-Mu 4020 1983 5 sh1-Mu 1746 1983 5 a2-Mum (Independent isolates - 1, 2, 3, 4, 1983 5 & 65, 6, 7, 8.) a2-Mum (Independent isolates - 1, 2, 3.) 1983 6 sh1-Mu 9026 1983 6 bz1-Mum9 1983-84 6 a1-Mus (Independent isolates - 1, 2, 3.) 1984 6 a1-Mum2 1984 6 a1-Mum3 1984 6 wx1-Mum1 1984 6 wx1-Mus2 (3 lines from a 13 kernel sector) 1984 7 wx1-Mus3 1984 7 wx1-Mus 181 (From a 29 kernel sector) 1984 7 wx1-Mum3 1984 7 wx1-Mus 215 1984 7 bz1-Mum10 (Original isolate) 1984 7 o2-Mum1 1984 7 wx1-Mum1 1984 7 wx1-Mum2 1984 7 sh1-Mu 4020 1984 7 sh1-Mu 9552 1984 7 bz1-Mum11 1984 7 a2-Mum3532 = a2-Mum4 1984 7 bz1-Mum5 1984 7 bz1-Mum6 1984 7 vp1-Mum1 1984 7 sh1-Mu 3017 1984 7 o?-Mu 3147 (Tested not to be allelic to o2) 1984 7 o?-Mu 3015 (Tested not to be allelic to o2) 1984 7 bz1-Mum (Independent isolates - 1, 2, 3, 4, 1984 7 & 8 5, 6, 7, 8, 9.) a2-Mum1 1984 8 a2-Mum2 1984 8 wx1-Mum2 1984 8 wx1-Mum1 1984-85 8 o2-Mum1 1984-85 8 o2-Mum3b 1984-85 8 c2-Mum1 1984 8 y1 wx1 gl1 Mum1 1984-85 8 y1 wx1 gl1 bt2 1982-83 8 bz1-Mum12 = bz1-Mum11 (2 bz1's from sibling 1983-84 8 plants.) y1 wx1 gl1 1985 8 sh1-Mu 9552 1984 8 sh1-Mu 4020 1984 8 (C1 sh1 bz1 wx1) X (sh1-Mu 1746/+) 1985 8 Mu-sh1 1746 1985 8 wx1-Mum2 1985 8 wx1-Mum1 1985 8 bz1-Mum11 1985 8 a1-Mum2 (Intense mutability-inbred stock.) 1985 8 a1-Mum4 (Aleurone mutable 5327-11.) 1985 8 wx1-Mu [New wx1 mutant from o.p. block = 1985 8 y1 wx1 gl1/Mu non-wx (Wx).] a1-Mum5 (Aleurone mutant 6056-8.) 1985 8 Pl. aleur./Dap2 1985 8 y1 wx-Mum2 gl1 1985 8 wx1-Mum5 1985 8 y1 wx1 bt2 1985 8 a2- Mus (Independent isolates - 1, 2, 3.) 1985 8 wx1-Mum3 1985 9 wx1-Mum4 1985 9 bz1-Mum10 1985 9 a2-Mum3 1985 9 o2-Mus3b 1985 9 y1 wx1 gl1 bt2/y1 wx1 gl1 bt2-Mu 9626 1985 9 y1 wx1 gl1 bt2 1985 9 bz1-Mum (Independent isolates - 2, 3, 4.) 1985 9 sh-Mu 3017 1985 9 bz1-Mum (Independent isolates - 1, 6, 7, 8, 1985 9 9, 10.) bz1-Mum11 1985 10 bz1-Mum12 (May be an allele to bz1-Mum11.) 1985 10 a2-Mum1 1985 10 a2-Mum2 1985 10 y1 wx1 gl8 1985 10 I.S.U. yg2 1985 10 y1 wx1 sh1 gl1 1985 10 a1-Mum (Independent isolates - 1, 2, 3.) 1985 10 y1 gl1 wx-Mum2 1985 10 wx1-Mum6 1985 10 wx1-Mum7 1985 10 vp5-Mum 3267-4 1985 10 vp9-Mu 3311-35 1985 10 y1 wx1 gl1 1985 10 Pl. Aleur./a1-Mum4 1985 10 bz1-Mus (Independent isolates - 1, 2, 3, 4, 1982 10 5, 6, 7, 8, 9, 10.) Pl. Aleur./a2-Mus1 1983 10 Pl. Aleur./a2-Mus2 1983 10 Pl. Aleur./a2-Mus3 1983 10 Pl. Aleur./a1-Mum5 1985 10 Stand./wx1-Mum6 1985 10 Stand./wx1-Mum7 1985 10 bz1-Mus1 X Pl. Aleur. Mutator & selfed 1985 10 (segregated mutable kernels.) (Ditto for independent isolates of 1985 10 bz1-Mus2, 3, 4, 5, 6, 7, 10.) c2-Mum1 1986 11 a1-Mum1 1986 11 a1-Mum2 (Good mutable) 1986 11 a1-Mum3 (Good mutable) 1986 11 y1 wx1 su1 gl1 1986 11 y1 wx1 bt2 gl1 1986 11 sh1-Mu6 1986 11 y1 bt2 wx1-Mum2 1986 11 bz1-Mum11 1986 11 a1-Mum4 1986 11 y1 wx1-Mum6 1986 11 y1 wx1-Mum7 1986 11 a1-Mum5 1986 11 Dap2 1986 11 wx1-Mum (Independent isolates - 5, 8, 10, 1986 11 11.) bz1-Mum10 1986 11 fl2-Mu 9234 1986 12 wx1-mum3 1986 12 vp5-Mu 3076-36 1986 12 vp9-Mu 3111-35 1986 12 Dap1/W22 Rscm2 & reciprocal 1986 12 y1-w mut (Not Mutator induced, self- „ 1986 12 pollinated & outcrossed to Stand. „ From Bikini atom bomb tests.) „ sh1-Mu 8423-1 „ 1986 12 „ sh1-Mu 8423.1-1 „(Independent new sh1 mutants)1986 12 „ sh1-Mu 8423.1-2 „ 1986 12 I.S.U. yg2 1986 12 (sh1 bz1 x2 del/sh1 bz1 x3 del) (Self- 1986 12 pollinated.) (sh1 bz1 x2 del/sh1 bz1 x3 del) X Pl. Aleur. 1986 12 (sh1 bz1 x2 del/sh1 bz1 x3 del) X c1 sh1 bz1 wx1 1986 12 Dap1 1986 12 a2-Mus (Independent isolates - 1, 2, 3. 1986 12 Self-pollinated & X Pl. Aleur.) bz1-Mum (Independent isolates - 2, 3, 4, 5, 1986 12 8, 9. Self-pollinated & X Pl. Aleur.) a2-Mum1 (Self-pollinated & X Pl. Aleur.) 1986 12 a2-Mum2 (Self-pollinated & X Pl. Aleur.) 1986 12 wx1-Mu16 1986 13 Stand./ wx1 del. C34 1986 13 l*-4920 1986-87 13 vp5-Mu 3076 1986-87 13 vp9-Mu 3111 1986-87 13 wx1-Mum (Independent isolates - 5, 6, 7, 1986-87 13 8, 9, 10, 11.) sh1-Mu 1746 1986-87 13 wx1-Mum1 1986-87 13 I.S.U. yg2 1986 13 Putative chrom. 3 del. (Linked to a1 locus.) 1986 13 y1 wx1 Mum5 1986 13 y1 wx1 Mum10 1986 13 y1 wx1 Mum11 1986 13 ps-Mu 1105 1986 13 Stand./vp5-Mu 3076 1986 13 Stand./vp9-mu3111 1986 13 I.S.U. y9 1987 13 (Stand./gl15 bm4) X Bf1 bm4 1987 13 Pl. Aleur./a1-x-1 1987 13 Pl. Aleur./a1-x-3 1987 13 a1-Mum2 (Intense nutability. Outcrossed 1987 13 three times to a1 sh2.) a1-Mum3 (Intense mutability. Outcrossed) 1987 13 three times to a1 sh2.) y1 gl1 wx1-Mum3 1987 13 sh1 bz1 x2 deletion 1987 13 sh1 bz1 x3 deletion 1987 14 bz1-Mum3 1987 14 bz1-Mum4 1987 14 wx1 R-scm2 1987 14 y1 (gl1) wx1-Mum9 1987 14 y1 gl1 wx1-Mum10 1987 14 y1 gl1 wx-Mum11 1987 14 bz1-Mum15 1987 14 bz1-Mum16 1987 14 y1 gl1 wx1-Mum5 1987 14 y1 wx1 gl1 1987 14 y1 wx1 gl8 1987 14 vp5-Mum 3076 1987 14 vp9-Mum 3111 1987 14 y1-8549 (pastel seedlings) 1987-88 14 a2-Mus1 1986 14 a2-Mus2 1986 14 sh2-Mu deletion 1987 14 vp5-Mumm1 (An allele obtained from Dr. Mumm.) 1980 14 vp5-Mumm2 (An allele obtained from Dr. Mumm.) 1987 14 (lw2-7752/+) X (w3/+) 1987 14 (w3-Everett #1/+) X (w3/+) 1987 14 Stand./al-Brawn (Homozygous) 1977 14 a1 wx1 1988 14 yg2-Mum (Independent isolates - 1, 2, 3, 4, 1986 14 5, 6, 7, 8, 9.) Pl. wx1/a1 wx1 1988 14 an-bz2 deletion 1988 14 y1-pastel Casper 1988 14 lw1-Mum 3108 1988 14 al-Brawn 1988 14 dek13 1988 14 cl1 cl1 Clm3 Clm3 1988 14 cl1/cl1-7716 Clm3 Clm3 1988 14 a1-x1 deletion 1988 15 wx1-Mum2(Mutant with large reverent sectors.)1988 15 Stand./wx1-Mum14 1988 15 Except where indicated otherwise, the following stocks have been stored at room temperature until the summer of 1993, when they were transferred to cold storage. Stamd./wx1-Mum14 1987 15 Stamd./wx1-Mum15 1987 15 Bf1-Mu 044-4 (Deletion-uncovers Bf1 and bm4.)1985-86 15 (In cold storage until 1988.) cl1-w7716 cl1-7716 Clm3 Clm3 (In cold storage 1979 15 until 1988.) vp1-Mum3 1987 15 vp9-Mum2 (3111) 1988 15 Dap-py 1988 15 y9 oy 1988-89 15 vp9-Mum2 (3111) 1988-89 15 vp1-Mc. 1987 15 a1 bm1 bt1/a1-Mum3 1988 15 a1 wx1 1988 15 Bf1-Mu 044-4 deletion/bm4 (Deletion uncovers 1986 15 bm4.) Bf1-Mu 046-6/bm4 (Possible deletion that 1986 15 uncovers bm4.) B1f-Mu 046-6/bm4 (Possible deletion that 1987 15 uncovers bm4.) ISU yg2 1989 15 (ms-Hooker/OH43) (On 9L, Self pollinated.) 1988 15 (ms-Bear #2/OH43) (On 9L, Self pollinated.) 1988 15 a1-wx (White kernels with germs with the 1989 15 surface, but not the rest of the scutellum, pigmented purple.) a1 sh1/a1 wx1 (The a1 wx1 is the same as the 1989 15 one above. This F1 does not have germs with pigmented surfaces.) a1 wx1 (Ear from sibling plant of the one 1989 15with germs with pigmented surface,but these have colorless germs.) Pl. aleur./a1 wx1 (a1 wx1 plant is one from 1989 15 above with colorless germs.) vp9-Mum3 1988 15 (Pl. wx1/a1 wx1) X a1 wx1 1988-89 15 bz1-Mum18 1990 15 l10-Mus 1359 1990 15 (dek) l*-1104-Mum 1990 15 a1 wx1 1990 16 pyd/+ (Self-pollinated. Original F1 stock 1991 16 supplied by Dr. Mc Clintock.) I.S.U. yg2 1991 16 (v28)/+ (Self-pollinated) 1991 16 Pl. Aleur. X (pyd/+) (pyd F1 supplied by Dr. 1991 16 Mc Clintock.) Stand. X (pyd/+) (pyd F1 supplied by Dr. 1991 16 Mc Clintock.) Stand. X (v28/+) 1991 16 pyd/+ (Original pyd stocks obtained 1990 16 supplied by Dr. Mc Clintock.) THE FOLLOWING STOCKS HAVE BEEN RETAINED AT IOWA STATE UNIV. (Stocks listed below have been in cold storage.) The stocks used to initiate the systematic 1971 1 study of the Mutator system. Ditto 1972 1 Mutator stock [Sibling plant(s) segregated 1974 1 for a new mutant.] Pl. Mutator stock (Tested positive for Mutator 1974 1 activity.) Mutator stock [Sibling plant(s) segregated 1975 1 for a new mutant.] Mutator stock (Tested positive for Mutator 1975 1 activity.) Mutator stock [Sibling plant(s) segregated 1976 2 for a new mutant.] Mutator stock (Tested positive for Mutator 1976 2 activity.) Pl. Mutator stock [Sibling plant(s) seg- 1977 regated for a new mutant.] Mu2 (Not tested for Mutator activity.) 1977 2 Mutator stock (Tested positive for Mutator 1977 3 activity.) Mutator stock [Sibling plant(s) segregated 1977-78 3 for a new mutant.] Mutator stock (Tested positive for Mutator 1977-78 3 activity.) Mu2 (Tested positive for Mutator activity.) 1977-78 3 Mutator stock (Tested positive for Mutator 1978 3 activity.) lw2-7752 1978 3 Mu2 (Tested positive for Mutator activity.) 1978 3 Mu4 (Tested positive for Mutator activity.) 1978-79 3 Mutator stock (Tested positive for Mutator 1978-79 3 activity.) Mutator stock (Tested positive for Mutator 1979 3 activity.) Pl. Mutator stock (Not tested for Mutator 1979 3 activity.) y1 wx1 gl1 Mutator stock [Sibling plant(s) 1979 3 segregated for a new mutants.] y1 wx1 Mutator stock (Tested positive for 1979 3 Mutator activity.) B76 (Inbred) 1979 3 B73 (Inbred) 1979 3 A632 (Inbred) 1979 3 MO17 (Inbred) 1979 3 y1 wx1 1979 3 y1 wx1 gl1 1979 3 Mutator stock [Sibling plant(s) segregated 1979-80 4 for a new mutant.] y1 wx1 gl1 Mutator stock (Tested positive 1979-80 4 for Mutator activity.) Mutator stock [Sibling plant(s) segregated 1979-80 4 for a new mutant.] Mutator stock [Reciprocal crosses to Stand. 1980 4 Sibling plant(s) segregated for a new mutant.] Mutator stock [Sibling plant(s) segregated 1980 4 for a new mutant.] Mu16 (Mu8 X Mu8) [Sibling plant(s) of one 1980 4 parent segregated for a new mutant.] y1 wx1 Mutator stock (Tested positive for 1980 4 Mutator activity.) Mutator stock (Tested positive for Mutator 1980 5 activity.) CI 187-2 (Inbred) 1981 5 M14 (Inbred) 1981 5 OH43 (Inbred) 1981 5 N25 (Inbred) 1981 5 Mu2 o. c. (Tested positive for Mutator 1978 5 activity.) Mu2 o. c. (Tested positive for Mutator 1978-79 5 activity.) Mutator stock (Tested positive for Mutator 1978-79 5 activity.) Mutator stock (Tested positive for Mutator 1980 5 activity.) Mutator stock (Tested positive for Mutator 1980-81 5 activity.) Mutator stock (Tested positive for Mutator 1981-82 5 activity.) Mutator stock (Tested positive for Mutator 1978 5 activity.) Mutator stock (Tested positive for Mutator 1981 5 activity.) Pl. Aleur. Mutator stock (Tested positive 1977 5 for Mutator activity.) Pl. Aleur. Mutator stock (Tested positive 1979 5 for Mutator activity.) Pl. Aleur./homo. a1-Mum1 1981-82 5 Pl. Aleur./homo. a1-Mum2 1981-82 5 Pl. Aleur./homo. a1-Mum3 1981-82 5 a1 sh1/homo. a1-Mum1 1981-82 5 a1 sh1/homo. a1-Mum3 1981-82 5 Pl. Aleur./A1 A2 c1 r1 Mutator stock (Tested 1981-82 5 positive for Mutator activity.) Mutator stock (Tested positive for Mutator 1982 5 activity.) Pl. Aleur./A1 A2 c1 r1 Mutator stock (Tested 1982 5 positive for Mutator activity.) Mu4 o. c. (Tested positive for Mutator 1982 5 activity.) bz1-Mum2 (Outcrossed to Pl. Aleur.) 1982 5 bz1-Mum3 (Outcrossed to c1 sh1 bz1 wx1) 1982 5 bz1-Mum4 (Outcrossed to Pl. Aleur.) 1982 5 Mutator stock (Tested positive for Mutator 1982-83 5 activity.) Mu2 (Tested positive for Mutator activity.) 1982 5 Mu2 o. c. (Tested positive for Mutator 1982 5 activity.) Mu4 o. c. (Tested positive for Mutator 1982 5 activity.) Mu3 (Tested positive for Mutator activity.) 1982-83 5 Bf1 (Homozygous) 1980 6 Mu4 (Outcrossed two times. Tested positive 1982 6 for Mutator activity.) a2-Mum2 1982 6 W22 R-scm2/bz1-Mum1 (Self-pollinated.) 1982-83 6 Mu2 (Outcrossed two times. Tested positive 1982 6 for Mutator activity.) Mu3 (Mu2/Mu1) (Tested positive for Mutator 1982 6 activity.) Mu6 (Mu4/Mu2) (Tested positive for Mutator 1982 6 activity.) Mu16 (Mu8/Mu8) (Tested positive for Mutator 1982 6 activity.) Mu12 (Mu8/Mu4) (Tested positive for Mutator 1982 6 activity.) Mu9 (Mu8/Mu1) (Tested positive for Mutator 1982 6 activity.) Mu2 (Outcrossed three times. Tested 1982-83 6 positive for Mutator activity.) Mu4 (Outcrossed two times. Tested 1982 6 positive for Mutator activity.) Mu8 (Outcrossed two times. Tested 1982 6 positive for Mutator activity.) Mutator stock (Tested positive for Mutator 1979 6 activity.) y1 wx1 1981 6 ISZP (Freeling's inbred)/Mu [Sibling plant(s) 1981 6 segregated for a new mutant.] Mutator stock [Outcrossed. Sibling plant(s) 1981 6 segregated for a new mutant.] ISZP (Freeling's inbred that supports 1981 6 Mutator activity.) N25 (Inbred) 1981 6 B55 (Inbred) 1981 6 CI-187-2 (Inbred) 1981 6 OH43 (Inbred) 1981 6 wx1 T4-9 6567/Mu [Sibling plant(s) segre- 1981 6 gated for a new mutant.] Mutator stock [Sibling plant(s) segregated 1981 7 for a new mutant.] A6328/Mu [(Sibling plant(s) segregated for 1981 7 a new mutant.] A6324/Mu [Sibling plant(s) segregated for 1981 7 a new mutant.] A6326/Mu [Sibling plant(s) segregated for 1981 7 a new mutant.] y1 wx1 gl1 Mutator stock (Untested?) 1981 7 Mutator stock [Sibling plant(s) segregated 1981-82 7 for a new mutant.] A6325/Mu [Sibling plant(s) segregated for 1981-82 7 for a new mutant.] Mu4 (Mu2/Mu2) (Untested) 1982 7 Mu2 (Outcrossed, untested) 1982 7 Pl. Aleur.2/Mu [Sibling plant(s) segregated 1982 7 for a new mutant.] A632 (Inbred) 1982 7 MO17 (Inbred) 1982 7 B76 (Inbred) 1982 7 B73 (Inbred) 1982 7 Mu4 [Outcrossed two times. Sibling 1982 8 plant(s) segregated for a new mutant.] Mu2 [Outcrossed two times. Sibling 1982 8 plant(s) segregated for a new mutant.] Mutator stock [Sibling plant(s) segregated 1982 8 segregated for a new mutant.] Mutator Stock [Sibling plant(s) segregated 1982 9 segregated for a new mutant.] Q66 (Inbred) 1982 9 Q67 (Inbred) 1982 9 B77 (Inbred) 1982 9 B79 (Inbred) 1982 9 M14 (Inbred) 1982 9 W22 (Inbred) 1982 9 Mu2 o. c. [Sibling plant(s) segregated for 1982 9 a new mutant.] (yg2-Mu /yg2) X Stand.(Independent isolates - 1982 9 104-8, 106-2, 103-1, 106-4, 101-4, 102-4) [Pl. Aleur/(+)]/Mu (Tested positive for 1983 9 Mutator activity.) A6323/Mu (Tested positive for Mutator 1977-78 9 activity.) Pl. Aleur Mutator stock (Tested positive 1982-83 9 for Mutator activity.) Mu4 [Outcrossed three times. Sibling 1982-83 9 plant(s) segregated for a new mutant.] bz1-Mum (Independent isolates - 1, 3, 4.) 1982-83 9 a1-Mum (Independent isolates - 1, 2, 3.) 1982-83 9 Mu2 [Outcrossed. Sibling plant(s) 1983 9 segregated for a new mutant.] Mu3 [Outcrossed. Sibling plant(s) 1983 10segregated for a new mutant.] Mutator stock [Sibling plant(s) segregated 1983 10 segregated for a new mutant.] Mu2 [Outcrossed. Sibling plant(s) 1983 10 segregated for a new mutant.] Mu4 [Outcrossed. Sibling plant(s) 1983 10 segregated for a new mutant.] Mu4 [Outcrossed three times. Sibling 1983 10 plant(s) segregated for a new mutant.] Mu2 [Outcrossed three times. Sibling 1983 10 plant(s) segregated for a new mutant.] Mu3 (Outcrossed. Sibling plant(s) 1983 10 segregated for a new mutant.) Mu4 (Outcrossed three times. Sibling 1983 10 plant(s) segregated for a new mutant.) sh1-Mu 9026 1983 10 yg2-Mu/Stand. (Plus a few reciprocal crosses 1983 10 and self pollinations. Approximately 80 different independent yg2-Mu isolates.) Ditto (Continuation of the previous 1983 11 stocks.) yg2-Mu/Stand. (Reciprocal crosses of several 1983-84 11 independent isolated X yg2.) Ditto (Continuation of above stocks.) 1983-84 12 Mu2 (Tested positive for Mutator activity.) 1983 12 Mu6 (Mu2 X Mu4) (Tested positive for Mutator 1983 12 activity.) Mu5 (Mu2 X Mu3) (Tested positive for Mutator 1983 12 activity.) Mu4 (Mu2 X Mu2) (Tested positive for Mutator 1982-83 12 activity.) Mutator Stock (Tested positive for Mutator 1980 12 activity.) Mu2 (Outcrossed) (Tested positive for 1982-93 12 Mutator activity.) Mu4 (Outcrossed) (Tested positive for 1979 12 Mutator activity.) Mu4 (Mu2 X Mu2) (Tested positive for 1982-83 12 Mutator activity.) Mu6 (Mu3 X Mu3) (Tested positive for 1983 12 Mutator activity.) yg2-Mu /Stand. (Independent isolates- 104-1, 1983 12 108-1, 109-4, 109-5, 110-5, 110-7, 110-10, 111-9, 112-2, 113-10, 114-5, 114-10, 115-1, 115-5, 116-2, 116-4, 116-8.) bz1-Mum (Independent isolates -1, 2, 3, 1983 12 4, 5, 6, 7, 8, 9.) Mutator Stock (Tested positive for Mutator 1983 12 activity.) Mu6 (Outcrossed. Tested positive for 1983 12 Mutator activity.) Pl Aleur. Mu2 (Tested positive for Mutator 1983-84 12 activity.) Mu2 (Outcrossed. Tested positive for 1983 12 Mutator activity.) Mu4 (Outcrossed. Tested positive for 1983 12 Mutator activity.) Q66 (Inbred) 1982 12 Q67 (Inbred) 1982 12 B77 (Inbred) 1982 12 B79 (Inbred) 1982 12 Mu4 (Outcrossed. Tested positive for 1983-84 12 Mutator activity.) Mu3 (Outcrossed. Tested positive for 1983-84 12 Mutator activity.) Mu5 (1 X 4) (Tested positive for Mutator 1983-84 12 activity.) Mu5 (2 X 3) (Tested positive for Mutator 1983-84 12 activity.) Mu3 [Outcrossed. Sibling plant(s) 1984 12 segregated for a new mutant.] (Ditto) 1984 13 Mu6 (3 X 3) [Outcrossed. Sibling plant(s) 1984 13 segregated for a new mutant.] bz1-Mum9 1984 13 a1-Mus (Independent isolates - 1, 2, 3.) 1984 13 a1-Mum2 1984 13 a1-Mum3 1984 13 wx1-Mum1 1984 13 y1 Wx1 Mutator stock (Tested positive for 1981 13 Mutator activity.) wx1-Mus2 (Three lines from a 13 kernel 1984 13 sector.) wx-Mus3 1984 13 wx-Mus 181 (From a 29 kernel sector) 1984 13 wx1-Mum3 1984 13 y1 wx1 gl1 Mutator stock (Tested positive 1984 13 for Mutator activity.) Pl. Aleur. Mutator stock (Tested positive 1984 13 for Mutator activity.) yg2-Mu /Stand. (Independent isolates - 1983 13 106-3, 106-6, 115-2.) Mutator Stock (Tested positive for Mutator 1982 13 activity.) Pl. Aleur/Mu2 Pl. Aleur [Sibling plant(s) 1984 13 segregated for a new mutant.] Stand./Mu8 (3 X/4 X 1/) [Sibling plant(s) 1984 13 segregated for a new mutant.] yg2-Mu 106-3 X yg2/Stand. 1984 13 bz1-Mum11 1984 14 yg2-Mu 106-5 X yg2/Stand. 1984 14 Mutator Stock [Sibling plant(s) segregated 1984 14 a new mutant.] Mu2 [Outcrossed two times. Sibling plant(s) 1984 14 segregated for a new mutant.] Mu4 [Outcrossed two times. Sibling plant(s) 1984 14 segregated for a new mutant.] [(yg2-Mu /yg2) X Stand]./yg2 Stocks and a „ few reciprocal crosses of the yg2-Mu/yg2 „ X Stand with yg2. A few of these yg2-Mu „ stocks were yg2-Mum mutants. The „ following is a list of the isolates. Some „ gave positive test for the presence of „ deletions, indicated by (del.) Others gave „ doubtful tests for deletions, indicated by „ (del.?). Others gave no indication of a „ 1984 14 deletion. These are not followed by any „ designation. 104-1, 104-4 (mutable). „ 104-7 (del.), 108-1, 108-3 (del.), 108-8 „ (del.), 109-4 (del.?), 109-5, 109-9 (del.?),„ 110-5 (mutable), 110-7 (del.?), 110-8 „ (del.), 110-9 (del.), 110-10 (del.?), 111-9,„ 113-10, 114-1 (del.), 114-5, „ 1984 14 114-10 (del.),115-5 (del.?), 116-2,116-4, „ 1984 15 116-6 (del.?), 116-8, 116-10 (del.), „ Mu5 (Outcrossed five times. [Sibling plant(s) segregated for a new mutant.] 1984 15 Stand./Pl. Aleur. Mutator Stock (Sibling 1984 15 plant(s) segregated for a new mutant.] Mutator Stock [Sibling plant(s) segregated 1984 15 for a new mutant.] Pl. Aleur. Mutator/a1-Mum1 stable (Self- pollinated, segregated mutable 1984 15 kernels.) Pl. Aleur. Mutator/a1-Mum1 stable X a1 sh2 1984 15 (Segregated mutable kernels.) Pl. Aleur. Mutator/a1-Mum3 stable (Self- pollinated, segregated mutable 1984 15 kernels.) Pl. Aleur. Mutator Stock [Sibling plant(s) 1984 15 segregated for a new mutant.) Pl. Aleur. Mutator Stock (Tested positive 1983 15 for Mutator activity.) Mutator Stock (Tested positive for Mutator 1984 16 activity. Mu6 (2 X 4) (Outcrossed. Tested positive 1984 16 for Mutator activity.) Mu6 (3 X 3) (Outcrossed. Tested positive 1984 16 for Mutator activity.) Mu2 (Outcrossed. Tested positive for 1984 16 Mutator activity.) Mu5 (2 X 3) (Outcrossed. Tested positive 1984 16 for Mutator activity.) Mu4 (2 X 2) (Outcrossed. Tested positive 1984 16 for Mutator activity.) Mutator Stock (Outcrossed. Tested positive 1984 16 for Mutator activity.) Mu8 (4 X 4) (Outcrossed. Tested positive 1984 16 for Mutator activity.) Mu8 (3 X /2 X 3/) (Outcrossed. Tested 1984 16 positive for Mutator activity.) Mu8 (3 X /4 X 1/) (Outcrossed. Tested 1984 16 positive for Mutator activity.) Pl. Aleur. Mutator Stock (Outcrossed. 1984 16 tested positive for Mutator activity.) (yg2-Mu 108-8 X Stand.)/yg2 1984 16 (Stand./yg2-Mu) X yg2 (Independent isolates - 1984 16 101-4, 104-8, 106-3. Deletions.) Stand./(yg2-Mu 113-10) X yg2 (Negative 1984 16 deletion test.) Q66 (Inbred) 1984 16 B77 (Inbred) 1984 16 B79 (Inbred) 1984 16 a1-Mum (Independent isolates - 1, 2, 3.) 1985 16 bz1-Mum11 1985 16 Stand./(Bf1-Mu X Bf1) (Also a few reciprocal 1985 16 crosses. Over 150 isolates.) (Ditto) 1985 17 (Ditto) 1985 18 (Ditto) 1985 19 Pl. Aleur. Mutator Stock [Sibling plant(s) 1985 19 segregated for a new mutant.) Q66 (Inbred) 1985 19 Q67 (Inbred) 1985 19 (Bf1-Mu X Bf1 043-3)/Stand. 1985 19 a1-Mum2 (Intensely mutable inbred stock.) 1985 19 Mu2 (Outcrossed. Tested positive for 1985 19 Mutator activity.) a1-Mum2 1985-86 19 a1-Mum3 1985-86 19 a1-Mum3 1985-86 20 a1-Mum2 (Intensely mutable inbred stock.) 1985-86 20 Bf1-Mu (Independent isolates - 044-9, 1985-86 20 045-7, 548-1, 547-6, 044-4. Putative deletions.) Bf1 1985-86 20 (yg2-Mu X yg2)/Stand. (Independent isolates- 1983 20 108-8, 110-8, 110-9, 114-1, 117-8, 116-10, 104-7, 108-3, 110-6, 117-5. Deletions.) [(yg2-Mu 107-2 /yg2) X Stand.]/yg2 (Deletion) 1984 20 [(Bf1-Mu 842-7/Bf1) X Stand.]/Bf1 1985-86 20 (Bf1-Mu 543-8 X Bf1)/Stand. 1985 20 (Bf1-Mu 046-6 X Bf1)/Stand. 1985 20 (yg2-Mu107-4 X yg2)/Stand. (Deletion) 1983 20 [(yg2-Mu X yg2)/Stand.] X yg2 (Independent 1984 20 isolates - 102-1, 104-7, 107-2, 108-3, 112-2, 116-10, 117-5, 117-8, 115-2.) (Bf1-Mu X Bf1)/Stand. (Independent isolates- 1985 20 044-6, 044-9, 045-4, 045-6, 046-7, 047-10, 048-9, 049-9, 050-3, 544-8, 549-5.) wx1-Mum1 1984 20 Stand./(yg2-Mu X yg2) (Seven independent 1983 20 isolates.) Ditto (Eighty-two independent 1983 21 isolates.) Ditto (Ten independent 1983 22 isolates.) Pl Aleur Mutator Stock (Tested positive for 1985 22 Mutator activity.) Mu4 (2 X 2) Outcrossed (Tested positive for 1985 22 Mutator activity.) a1-Mus1 X Pl. Aleur. Mutator (Mutability not 1985 22 not induced.) a1-Mus2 X Pl. Aleur. Mutator (Mutability not 1985 22 not induced.) a1-Mus3 X Pl. Aleur. Mutator (Mutability not 1985 22 not induced.) a1-Mus4 X Pl. Aleur. Mutator (Mutability not 1985 22 not induced.) a2-Mus1 X Pl. Aleur. Mutator (Mutability not 1985 22 not induced.) a2-Mus2 X Pl. Aleur. Mutator (Mutability not 1985 22 not induced.) bz1-Mus4 X Pl. Aleur. Mutator (Mutability not 1985 22 not induced.) bz1-Mus8 X Pl. Aleur. Mutator (Mutability not 1985 22 not induced.) bz1-Mus9 X Pl. Aleur. Mutator (Mutability not 1985 22 not induced.) yg2/[Stand./(yg2-Mu X yg2)] (Independent 1986 22 isolates - 108-8, 110-8, 114-4, 117-8. wd deletions.) a1-Mum1 1986 22 a1-Mum2 (Intense mutability.) 1986 22 a1 sh2/a1-Mum2 (Putative 1:1 ratio.) 1986 22 a1-Mum3 (Intense mutability.) 1986 22 a1 sh2/a1-Mum3 (Putative 1:1 ratio) 1986 22 a1-Mum2 (Inbred stock. Intense mutability.) 1986 22 a1-Mum2 (Inbred stock. Loss of mutability.) 1986 22 [Pl. Aleur. Mutator/a1-Mum2 (Intense mutability.)] (Self- pollinated. Progeny intensely mutable.) 1986 22 [Pl. Aleur. Mutator/a1-Mum3 (Intense mutability.)] (Self- pollinated. Progeny intensely mutable.) 1986 22 a1-Mum5 1986 22 bz1-Mum10 1986 22 bz1-Mum10 1986 23 Mu2 Outcrossed [Sibling plant(s) segregated 1986 23 for a new mutant.] Bf1/[(Bf1-Mu X Bf1)/Stand.] (Independent 1986 23 548-1, 547-6, 044-4, 544-5, 547-9, 045-3, 050-8, 046-6.) Mutator Stock [Sibling plant(s) segregated 1986 23 for a new mutant.] yg2-Mu/wd1 (Independent isolates - 114-1, 1986 23 117-8, 116-10, 110-6, 117-5, 107-1. Deletions.) wd1 1986 23 a2-Mum1 (Self-pollinated and X Pl. Aleur.) 1986 23 a2-Mum2 (Self-pollinated and X Pl. Aleur.) 1986 23 (Bf1-Mu X Bf1/Stand.) X Bf1 and reciprocal 1986 23 crosses (Deletion, 17 different isolates.) (Bf1-Mu X Bf1/Stand.) X Bf1 and reciprocal 1986 24 crosses (Deletion, 2 different isolates.) a1 sh2/(Pl. Aleur. Mutator stock X a1-Mum2) 1986 24 and reciprocal crosses. (a1-Mum2 is intensely mutable.) Mutator Stock [Sibling plant(s) segregated 1986 24 segregated for a new mutant.] Putative (MuDR/+) X (a1-Mum2 Stable/+) (1:1) 1986 24 Putative (MuDR/+) X (a1-Mum3 Stable/+) (1:1) 1986 24 a1-Mum2 (Self-pollinated and X a1 sh2.) 1986 24 a1-Mum3 (Self-pollinated and X a1 sh2.) 1986 24 Mu2 [Sibling plant(s) of one or both parents 1986 24 segregated for a new mutant.] (Ditto) 1986 25 a1 sh2/a1-Mum2 1986 25 a1 sh2/a1-Mum3 1986 25 bz1-Mum11 1986 25 a1-Mum2 (Inbred, intensely mutable) 1986 25 Mu4 (Outcrossed. Tested positive for Mutator 1986 25 activity.) Pl. Aleur. Mutator Stock (Tested positive 1986 25 for Mutator activity.) Mutator Stock (Tested positive for Mutator 1986 25 activity.) Mu2 (Outcrossed. Tested positive for 1986 25 Mutator activity.) Mu4 (Outcrossed. Tested positive for 1986 25 Mutator activity.) a1 sh2/a1-Mum2 [Putative mutable:stable, 1986-87 25 (1:1).] Putative (MuDR/+) X (a1-Mum2/+) (1:1) 1986-87 25 a1-Mum2 (Outcrossed. Intensely mutable.) 1986-87 25 a1-Mum3 (Outcrossed. Intensely mutable.) 1986-87 25 (Ditto) 1986-87 26 a1-Mum2 (Inbred. Intensely mutable.) 1986-87 26 (a1-Mum2/+, MuDR) X a1 sh2 (1:1) 1986-87 26 a1-Mum3 [Outcrossed. Putative (1:1)] 1986-87 26 bz1-Mum11 (Self-pollinated and outcrossed 1986-87 26 X c1 sh1 bz1 wx1.) a1 sh2/[(a1 sh2 X a1-Mum2), mutable from 1986-87 26 (1:1).] A636 (Inbred) 1986-87 26 wd-Mu (Independent isolates - 1, 2, 3.) 1986 26 Pl. Aleur./a1-Mum2 (Intensely mutable. 1985-86 26 Tested positive for Mutator activity.) a1-Mum2 (Intensely mutable -> stable, 1986 26 Hi Loss.) a1-Mum2 (Hi Loss X intensely mutable 1986 26 a1-Mum2 stock.) wd-Mu (Independent isolates - 1, 2, 4, 5, 6.) 1986 26 (Putative MuDR/+) X [a1-Mum2 stable from 1986 27 a1-Mum2/+, (1:1)] a1-Mum2 (Intensely mutable. Inbred.) 1986 27 a1 sh2/a1-Mum2 (Putative mutable:stable, 1:1) 1986 27 a1-Mum2 (mutable)/a1-Mum2 (Hi Loss) 1986 27 a1 sh2/a1-Mum3 (Putative mutable:stable, 1:1) 1896 27 a1-Mum3 (Mutable)/a1-Mum3 (Hi Loss) 1986 27 (Bf1-Mu X Bf1)/Stand. (Independent isolates - 1985 27 044-9, 548-1, 547-6, 044-4, 544-5, 045-3, 050-8. Deletions.) a1-Mum3 (Intensely mutable) 1983 27 a1-Mum2 (Inbred. Intensely mutable.) 1986-87 27 a1-Mum2 (Inbred. Intensely mutable.) 1985 27 Pl. Aleur./a1-Mum2 1982-83 27 bz1-Mum (Independent isolates - 2, 3, 4.) 1986 27 y1 wx1-Mum (Independent isolates - 3, 5, 9, 1986 27 10, 11.) a1-Mum2 (Hi Loss) 1986 27 Q66 (Inbred) 1986-87 27 Q67 (Inbred) 1986-87 27 (Putative MuDR/+) X [a1-Mum2 stable from 1985-86 27 (1:1)/+] a1 sh2/(a1 sh2 X a1-Mum2) (Putative 1:1) 1986 27 a1 sh2/(a1 sh2 X a1-Mum3) (Putative 1:1) 1986 27 Mutator Stock (Tested positive for Mutator 1986 27 activity.) Pl. Aleur. Mutator Stock (Tested positive 1986 27 for Mutator activity.) a1 sh2/[a1 sh2 X a1-Mum2 stable (Reactivated 1986 27 by crossing with Pl. Aleur. Mutator Stock.)] a1 sh2/(a1 sh2 X a1-Mum3) (Putative 1;1) 1986 28 a1 sh2/(a1 sh2 X a1-Mum2) (Putative 1:1) 1986 28 Pl. Aleur. Mutator Stock (Tested positive 1986 28 for Mutator activity.) A188/Mutator Stock (Tested positive for 1986 28 Mutator activity.) B732/Mutator Stock (Tested positive for 1986 28 Mutator activity.) Mu2 (Outcrossed. Tested positive for Mutator 1986 28 activity.) Mu4 (Outcrossed. Tested positive for Mutator 1983-84 28 activity.) Mutator Stock (Tested positive for Mutator 1986 28 activity.) wd-Mu1 (Self-pollinated and X Stand.) 1987 28 wd-Mu2 (Self-pollinated, X yg2 and X Stand.) 1987 28 wd-Mu3 (Self-pollinated, X yg2 and X Stand.) 1987 28 wd-Mu4 (Self-pollinated, X yg2 and X Stand.) 1987 28 wd-Mu5 (Self-pollinated and X yg2.) 1987 28 wd-Mu6 X yg2 1987 28 a1 sh2/[a1-Mum2 Stable (from Hi Low) X 1987 28 a1-Mum2 intensely mutable] (Reciprocally crossed.) (Progeny Stable.) a1-Mum2 (Inbred. Intensely Mutable.) 1987 28 [(a1-Mum2 Hi Low X a1-Mum2 Hi Low) Stable] 1987 28 (Self Pollinated) (Ditto) 1987 29 [a1-Mum3 Stable from (1:1) X a1 sh2 (Segregant from 1:1)] (1:1) 1987 29 [(a1-Mum2 Hi Low X a1-Mum2 Hi Low) = 2 X 2 = 1] (Self-pollinated) 1987 29 (a1 sh2/a1-Mum2 2 X 2 = 1) (Stable) 1987 29 [Pl. Aleur./a1-Mum2 (Intensely Mutable)] 1987 29 Self Pollinated, Progeny intensely mutable. (Tested positive for Mutator activity.) (Ditto, but tested negative for Mutator 1987 29 activity.) wx1-Mum7 1987 29 Pl. Aleur. Mutator/a1-Mum3 (Stable kernel from 1987 29 ear with a 1:1 ratio.) [(a1 sh2/a1-Mum3) (1:1)] (Self-pollinated, 1987 29 ear segregated 3:1 mutable:stable.) (Ditto) 1987 30 [a1 sh2/mutable from a1-Mum2 (1:1)] (Observed 1987 30 ratios in the progeny - 3:1 or 1:1.) [a1 sh2/mutable from a1-Mum3 (1:1)] (Observed 1987 30 ratios in the progeny - 3:1 or 1:1.) [a1 sh2 segregant from a1-Mum3 (1:1)] X [a1-Mum2 stable from (1:1)] [(Observed ratio (1:1).] 1987 30 Pl. Aleur./gl15 bm4 (Sibling crosses.) 1987 30 (wx1-Mum2 reverent/wx1-Mum2) (Self 1987 30 Pollinated.) (wx1-Mum2 reverent/wx1-Mum2)/c1 sh1 bz1 wx1 1987 30 Bf1 1987 30 a1-Mum2 (Inbred. Intensely mutable.) 1987 30 Mutator Stocks [Sibling plant(s) segregated 1987 30 for a new mutant.] a1-Mum2 (Inbred. Intensely mutable. Tested 1987 30 positive for Mutator activity. Stock self-pollinated and crossed to a1 sh2.) a1-Mum2 (Inbred. Intensely mutable. Tested 1987 30 negative for Mutator activity. Stock self-pollinated and crossed to a1 sh2.) [a1 sh2 segregant from a1-Mum2 (1:1)] X [a1-Mum2 stable from (1:1)] (Crosses segregated 1987 30 (1:1) or (3:1). (Ditto) 1987 31 [a1 sh2 segregant from a1-Mum3 (1:1)] X [a1-Mum3 stable from (1:1)] Crosses segregated 1987 31 (1:1) or (3:1). [Mutable from a1-Mum2 (1:1)] X (a1 sh2 and 1987 31 reciprocal crosses.) [Progeny segregated (1:1) and (3:1)] (Ditto) 1987 32 (Ditto) 1987 33 [Mutable from a1-Mum3 (1:1) X (a1 sh2 and 1987 33 reciprocal crosses.) [Progeny segregated (1:1) and (3:1).] a1-Mum2 (Intensely mutable. Outcrossed three 1987 33 to a1 sh2.) a1-Mum3 (Intensely mutable. Outcrossed three 1987 34 to a1 sh2.) Pl. Aleur. (Homo.?) Mutator Stock [Sibling 1987 34 plant(s) segregated for a new mutant.] a1 sh2 1987 34 a2 bm1 bt1 1987 34 c1 sh1 bz1 wx1 1987 34 c1 sh1 wx1 1987 34 a1 1987 34 a2 1987 34 c2 1987 34 r1 1987 34 Mutator Stock/B762 [Sibling plant(s) 1987 34 segregated for a new mutant.] [Mutable from a1-Mum3 (1:1)] X (a1 sh2 and 1987 34 reciprocal crosses.) [Progeny segregated for (1:1) and (3:1) ratios.] [Mutable from a1-Mum2 (1:1)] X (a1 sh2 and 1987 34 reciprocal crosses.) [Progeny segregated for (1:1) and (3:1) ratios.] wx1-Rscm2 1987 34 y1 wx1 gl1 1987 34 y1 wx1 gl8 1987 34 a1 sh2/Mutable kernels from a1-Mum3 MuDR/+ 1987 34 [Progeny segregated (1:1).] Pl. Aleur. Mutator Stock (Tested positive for 1985 34 Mutator activity.) Pl. wx1 1987 34 Various a1-Mum3 (1:1) stocks. 1986 & 1987 34 Various a1-Mum2 (1:1) Stocks. 1985 & 1986 34 Mu2 (Outcrossed. Tested positive for 1985 35 Mutator activity.) Pl. Aleur.2/a1-Mum2 (a1-Mum2 intensely 1987 35 mutable but when self-pollinated produced ear with low mutability = Hi Low. Self-pollinated plant tested negative for Mutator activity.) Pl. Aleur.2/a1-Mum2 (a1-Mum2 intensely 1987 35 mutable and when self-pollinated retained intense mutability. Self-pollinated plant tested positive for Mutator activity.) Pl. Aleur.2/a1-Mum2 (a1-Mum2 intensely 1987 35 mutable but when self-pollinated produced ear with low mutability = Hi Low. Self- pollinated plant tested positive for Mutator activity.) Pl. Aleur./a1-Mum2 (a1-Mum2 intensely 1987 35 mutable and when self-pollinated retained intense mutability. Self-pollinated plant tested negative for Mutator activity.) Pl. Aleur. Mutator Stock (Tested positive 1987 35 Mutator activity.) Pl. Aleur./a1-Mum3 (a1-Mum3 intensely 1987 35 mutable from an (1:1) ear. Tested negative Mutator activity.) a1 sh2/a1-Mum2 (a1-Mum2 inbred, intensely 1987 35 mutable stock. Tested positive for Mutator activity.) a1 sh2/a1-Mum2 (a1-Mum2 inbred, intensely 1987 35 mutable stock. Tested negative for Mutator activity.) Pl. Aleur. wx1 1987-88 36 a1 sh2/(a1-Mum3, intensely mutable from self-1987-88 36 pollination of an intensely mutable kernel from a 1:1 ear.) Self-pollinated ears - segregated 3:1, outcrossed ears segregated 1:1 or 3:1 ratios. a1-Mum2 Stable X a1-Mum2 Stable (Offspring 1987-88 36 gave mutable kernels. Progeny inbred three additional generations producing an intensely mutable stock.) a1-Mum2 (Inbred intensely mutable stock.) 1987-88 36 Bf1-bm4-Mu 044-4 (Deletion) 1987-88 36 TB-1La 1980 36 TB-lSb 1980 36 TB-1Sb-2L 4464 1980 36 TB-3Sb 1980 36 TB-3La 1980 36 TB-4Sa 1980 36 TB-5La 1980 36 TB-7Lb 1980 36 TB-9Sb 1980 36 TB-9Lc 1980 36 TB-10L19 1980 36 TB-7Lb-4L 4698 1981 36 Tb-1La-5S 4081 1981 36 Mu-Bf1 (Deletions? Independent Isolates - 1985 36 046-1, 047-7, 048-3, 544-9, 546-5, 547-5, 545-2, 545-5, 046-5.) [a1 sh2/a1-Mum3 (1:1) intensely mutable] 1987-88 36 (1:1) yg2-Mum (Independent isolates - 1, 2, 3, 4, 1983 36 5, 6, 7, 8, 9, 10.) [a1 sh2/a1-Mum2 Intensely mutable from (1:1) 1988 36 and reciprocal crosses.] (Ratios in progenies 1:1, 3:1, 7:1.) [a1 sh2/a1-Mum3 Intensely mutable from (1:1) 1988 36 and reciprocal crosses.] (Ratio in progenies 1:1.) TB-1La/Stand. (TB present.) 1988 36 (Ditto) 1988 37 TB-1Sb/Stand. 1988 37 TB-3La-2S-6270 1988 37 TB-1Sb-2L 4464 1988 37 TB-3Sb 1988 37 TB-3La 1988 37 TB-4Sa 1988 37 TB-9Lc 1988 37 Bf1/Bf1-Mu (Deletions? Independent 1988 37 isolates - 045-8, 046-1, 047-4, 048-3, 544-9, 546-5, 547-5, 545-2.) TB-7Lb-4L 4698 1988 37 TB-1La-5S 8041 1988 37 TB-9Sb 1988 37 TB-6Lc 1988 37 a1-Mum2 Stable/a1-Mum3 Stable (Offspring 1988 37 gave mutable kernels. Inbred four additional generation producing stocks segregating for intensely mutable kernels.) a1-Mum2 (Intensely mutable stock, retained 1988 37 intense mutability for many generations of inbreeding.) a1-Mum2 (Intensely mutable stock, retained 1988 37 intense mutability of for several generations of inbreeding until this generation, which yielded stable kernels.) Out crosses of the two above stocks X a1 sh2 1988 37 (a1 sh2/a1-Mum3 Intensely mutable kernels 1988 37 from a 1:1 ear and reciprocal crosses.) (Produced ears with 1:1 ratios.) (Ditto) 1988 38 a1-Mum2 (Intensely mutable stocks, retained 1988 38 intense mutability for many generations of inbreeding.) a1-Mum2 (Intensely mutable stocks, retained 1988 38 intense mutability for several generations of inbreeding until this generation, which yielded stable kernels.) TB-7Sc (Homozygous and X Stand.) 1988 38 TB-6Sa (Homozygous and X Stand.) 1988 38 TB-8Lc (X Stand.) 1988 38 Pl. Aleur./Bf1 bm4 (Sibling crossed, 1988 38 segregate Bf1.) W22 R-Scm2/(c1 sh1 wx1 X Tb-9Sd) 1988 38 TB-7Lb 1988 38 TB-8La 1988 38 TB-10Sc 1988 38 TB-10L19 1988 38 TB-2Sb 1988 38 TB-4Lf 1988 38 yg2-Mum (Self-pollinated and X Stand. 1988 38 & 39 Independent isolates - 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13. All isolates retained mutability except isolates 8 and 12.) [a1-Mum2 (1:1) Stable/MuDR/+] (1:1) 1988 39 MuDR/+ 1988 39 a1-Mum2 (1:1)/putative homozygous MuDR 1988 39 Putative homozygous MuDR 1988 39 a1-Mum3 (1:1)/putative homozygous MuDR 1988 39 Q66 (Inbred) 1988 39 Q67 (Inbred) 1988 39 B77 (Inbred) 1988 39 B79 (Inbred) 1988 39 MO17/Mutator Stock ([Sibling plant(s) 1988 39 segregating for a new mutant.) lw2 1988 39 vp5 1988 40 al1 1988 40 w3 1988 40 o5 1988 40 y9 1988 40 a1-Mum3 mutable from (1:1)/a1 sh2 and 1988 40 reciprocal crosses.] [Progenies segregated (1;1), (3;1), and (7:1).] [a1-Mum2 mutable from (1:1)/a1 sh2 and 1988 40 reciprocal crosses.] [Progenies segregated (1:1), (3:1), and (7:1).] (Ditto) 1988 41 [a1-Mum3 mutable fro (1:1)/a1 sh2 and 1988 41 reciprocal crosses.] [Progenies segregated (1:1) and (3:1).] cl1 cl1 Clm3 Clm3 or cl1 cl1-7716 Clm3 Clm3 or 1988 41 or cl1-7716 cl1-7716 Clm3 Clm3 cl1 cl1 Clm3 Clm3 1988 41 [a1 sh2 /(a1-Mum2 mutable X Mu32) and 1988 41 reciprocal crosses.] (Stable kernels.) [a1-Mum3 stable from (1:1)/a1 sh2 segregant 1988 41 MuDR/+] [Progenies segregated (1:1), (3:1), and (7:1).] [a1 sh2/a1-Mum3 mutable from (1:1) and 1988 41 reciprocal crosses.] [Progenies segregate (1:1), (3:1), (7:1), and (15:1)] (Ditto) 1988 42 wd-Mu2/yg2 1988 42 yg2/wd-Mu4 1988 42 [a1 sh2/a1-Mum2 mutable from (1:1) and 1988 42 reciprocal crosses.] [Progenies segregate (1:1) and (3:1).] a1-Mum2 (Homozygous intensely mutable, 1988 42 self-pollinated and out crossed to Pl. Aleur.) wx1-Mum2 (Yields large reverent sectors) 1988 42 [Pl. Aleur X (a1-Mum2 - original isolate/a1 1988 42 sh1)] (Self-pollinated and X a1 sh2.) Except where indicated otherwise, the following stocks have been stored at room temperature until the summer of 1993, when they were transferred to cold storage. (a1 sh2/a1-Mum2, intensely mutable) (Self- 1987 42 pollinated progeny retained intense mutability tested positive for Mutator activity.) (Ditto above, except tested for 1987 42 negative for Mutator activity.) Mutator stock/B732 (Tested positive for 1987 42 Mutator activity.) [a1-Mum3 intensely mutable from (1:1)/a1 sh2] 1987 42 (Produced 1:1 progeny which retained intense mutability and tested negative for Mutator activity.) [Self-pollinated ear of a plant from an 1986 42 a1-Mum3 intensely mutable kernel from a (1:1) progeny.] [Self-pollinated ear segregated (3:1) and progeny tested negative for Mutator activity.] lw2/TB-5La (In cold storage until 1988.) 1983 43 y1 wx1/TB-6Lc (In cold storage until 1988.) 1980 43 o5/TB-7Lb (In cold storage until 1988.) 1983 43 (Ditto) 1980 43 TB-8La (In cold storage until 1988.) 1980 43 v A552 (In cold storage until 1988.) 1980 43 c1 sh1 wx1 (In cold storage until 1988.) 1984 43 (Ditto) 1985 43 (Ditto) 1986 43 y9/TB-10Sc (In cold storage until 1988.) 1980 43 r1/TB-10L19 (In cold storage until 1988.) 1980 43 yg2-Mum (Independent isolates - 11, 12, 13.) 1986 43 lw1/TB-1La (In cold storage until 1988.) 1980 43 Pl. Aleur./a1 sh2 1987 43 [a1 sh2 segregant from an (1:1) ear/a1-Mum3 1987 43 stable from an (1:1) ear] (Yielded 1:1 progeny, which segregated for intense mutability and tested negative for Mutator activity.) [a1-Mum3 intensely mutable from (1:1)/a1 sh2] 1987 43 (Segregated 1:1 and intense mutability. Tested negative for Mutator activity.) [a1 sh2/a1-Mum2 intensely mutable from(1:1)] 1987 43 (Segregated 1:1 and intense mutability, and tested negative for Mutator activity.) [a1 sh2 segregant from an (1:1) ear/a1-Mum3 1987 43 stable from an (1;1) ear] (Yielded 1:1 progeny, which segregated for intense mutability and tested positive for Mutator activity.) a2 bm1 bt1 1987 43 Stand./wd-Mu (Independent isolates - 1, 2, 1987 43 3, 4, 5.) (Pl. Aleur./a1 sh2) (Self-pollinated) 1987 43 Bf1-Mu (Deletions?) (Independent isolates -„ 1985-86 544-5, 050-8, 046-6, 045-8, 047-7, „ and 43 048-3, 043-7, 546-5) „ 1986 vp1-Mum3 1987 43 y1 gl1 wx1-Mum6 1987 43 bz1-Mus (Independent isolates - 1, 2, 7, 10.) 1985 43 a1 sh2/a1-Mum3 (1:1) (Tested positive for 1987 43 intense mutability and negative for Mutator activity.) [(Bf1-Mu/Bf1) X Stand.] (Independent 1985 43 & 44 isolates - 044-1, 044-2, 044-3, 044-5, 044-7, 044-8, 045-1, 045-2, 045-5, 046-2, and many more! Stocks were in cold storage until 1988.) Mutator Stock/Mo17 (Tested positive for 1986 44 Mutator activity. In cold storage until 1988.) wx1-Mum14 1988 44 wx1-Mum15 1988 44 Mutator Stock/B763 (Tested positive for 1988 44 Mutator activity.) [Pl. Aleur./a1-Mum2 (1:1) Plants from stable 1988 45 kernels utilized in this cross.] [Tested negative for Mutator activity and mutable kernels (i.e., only stable kernels).] [Pl. Aleur. Mutator Stock/a1-Mum2 (1:1) Plants 1988 45 from stable kernels of the same a1-Mum2 parent in the above cross.] (Tested positive for Mutator activity and segregated for intensely mutable kernels. The Pl. Aleur. Mutator parent tested positive for Mutator activity.) [Pl. Aleur./a1-Mum3 (1:1) Plants from 1988 45 intensely mutable kernels.] (Tested positive for intensely mutable kernels and negative for Mutator activity.) [a1-Mum3 (1:1) Plants from intensely mutable 1988 45 kernels/Plants from the progeny of a selfed Pl. Aleur. Mutator Stock, which tested negative for Mutator activity.] (Tested positive for intensely mutable kernels but negative for Mutator activity.) Pl. Aleur. Mutator Stock 1988 45 H100/Mutator stock (Tested positive for 1988 45 Mutator activity.) a1 sh2/a1-Mum2 Intensely mutable (Tested 1988 45 positive for intensely mutable kernels and positive for Mutator activity.) a1 sh2/a1-Mum2 Intensely mutable (Tested 1988 45 positive for intensely mutable kernels and negative for Mutator activity.) a1-Mum2 Intensely mutable (Self-pollinated 1988-89 45 and outcrossed to a1 sh2.) [a1-Mum2 (1:1) Plants from intensely mutable 1988-89 45 kernels/Plants from the progeny of a self-pollinated Pl. Aleur. Mutator stock, which tested negative for Mutator activity.] (Self-pollination yielded kernels with low or no mutability.) [a1-Mum3 (1:1) Plants from intensely mutable 1988-89 45 kernels/Plants from the progeny of a self- pollinated Pl. Aleur. Mutator activity.] (Self-pollination yielded kernels with low or no mutability.) [a1-Mum3 (1:1) Plants from intensely mutable 1988-89 45 kernels/Plants from the progeny of a self- pollinated Pl. Aleur. Mutator activity.] (Self-pollination yielded kernels with intense mutability.) H100 (Inbred) 1988-89 45 [a1-Mum2 intensely mutable (Self-pollinated 1988-89 45 and X a1 sh2)] (Both self and outcross yielded intensely mutable kernels.) [a1-Mum2 intensely mutable (Self-pollinated 1988-89 45 and X a1 sh2)] (Both self and outcross yielded low mutable or stable kernels.) (Pl. Aleur./a1 sh2) (Self-pollinated) 1988-89 45 TB-9Sd/W22 R-Scm2 1988 45 TB-7Sc/Stand. 1988-89 45 a1 sh2/a1-Mum2 (1:1) (Sectored ear. Sector 1987 45 with 3:1 ratio and sector with only intensely mutable kernels.) ae 1987 45 Self-pollinated ear of[(Bf1-Mu X Bf1)/Stand.] 1988 45 (Progeny of the selfs segregated few or or not Bf1 seedlings. From the following independent isolates: 044-1, 044-5, 044-7, 044-8, 045-1, 045-2, 045-3, 046-3, 046-9, 047-1, 047-8, 048-1, 048-6, 049-1, 049-4, 049-7, 050-1, 050-4, 534-7, 544-1, 544-2, 544-7, 545-4, 545-7, 048-7, 048-4, 049-3, 048-5, 048-8, 048-11, 049-10, 050-9. (Continued list from above tests.) 1988 46 Isolates: 546-2, 546-6, 546-8, 546-9, 548-8, 548-8, 549-8, 550-5, 550-6, 550-7. (Bf1-Mu/TB-9Lc) Bf plant (hyperploid)/Stand. 1988 46 (Several different Bf1-Mu isolates.) (yg2-Mu/yg2 X Stand.) X yg2 (Reciprocal 1983 46 crosses of the following independent isolates: 101-2, 101-5, 101-8102-2, 103-2, 103-8, 106-7, 111-8, 115-10, 117-3, 117-4. Stocks in cold storage until 1988.) [a1-Mum2 (1:1) Plants from intensely mutable 1988 46 kernels/Plants from the progeny of a self- pollinated Pl. Aleur. Mutator stock, which tested negative for Mutator activity.] (Crosses tested negative for Mutator activity and segregated for mostly intensely mutable kernels.) [a1-Mum2 (1:1) Plants from intensely mutable 1988 46 kernels/Pl. Aleur.] (Same a1-Mum2 (1:1) stocks as above. Crosses tested negative for Mutator activity and segregated mostly for mostly intensely mutable kernels.) [a1-Mum2 (1:1) Plants from intensely mutable 1988 46 kernels/Plants from the progeny of a self- pollinated Pl. Aleur. Mutator stock, which tested negative for Mutator activity.] (Crosses tested negative for Mutator activity and segregated for mostly intensely mutable kernels.) [a1-Mum2 (1:1) Plants from intensely mutable 1988 46 kernels/Pl. Aleur.] (Same a1-Mum2 (1:1) stocks as above. Crosses tested negative for Mutator activity and segregated kernels with low or no mutability.) [a1-Mum2 (1:1) Plants from intensely mutable 1988 46 kernels/Plants from the progeny of a self pollinated Pl. Aleur. Mutator stock, which tested negative for Mutator activity.] (Crosses tested positive for Mutator activity and segregated for mostly intensely mutable kernels.) [a1-Mum2 (1:1) Plants from intensely mutable 1988 46 kernels/Pl. Aleur.] (Same a1-Mum2 (1:1) stocks as above. Crosses tested negative for Mutator activity and segregated kernels with low or no mutability.) Pl. Aleur. Mutator Stock (Tested positive 1985-86 46 for Mutator activity. In cold storage until 1989.) a1 sh2/a1-Mum2 (1:1) (In cold storage until 1986 46 1989.) bz1-Mum9 Stable/Homozygous MuDR from a1-Mum2 1988 46 stock.) [a1 sh2/a1-Mum2 (1:1) intensely mutable 1988 46 kernels] (1:1) a1 wx1 1988 46 a1/TB-3La 1988 46 c2/TB-1Sb-2L-4464-4Lf (Double compound TB.) 1988 46 Pl. Aleur wx1/TB-5Sc 1988-89 46 (Bf1-Mu X Bf1)/Stand. [Independent isolates - 1985 46 550-8, 5327-2, 5327-3, 5327-4, 5327-5, 5327-6, 5327-7, 5327-8, 5327-9, 5327-10, 5328-1, 5328-2, 5328-8, 5328-4, 5328-5, 5328-6, 5328-7, 5329-1, 5329-2, 5329-3, 5329-4, 5329-5, 5329-6, 5329-8, 5329-9, 5329-10, 5330-1, 5330-2, 5330-4, 5330-5, 5330-6, 5331-1, 5331-2, 5331-3, 5331-4, 5331-5, 5331-6, 5331-7, 5331-8, 5332-1, (Continuation of the previous stocks.) 5332-2, 5332-3, 5332-4, 5332-5, 5332-6, 5332-7. (Stocks were in cold storage until 1989.)] (Ditto, isolates continued.) 1985 47 [5332-8, 5332-9, 5333-3, 5333-4, 5333-4, 5333-5, 5333-6, 5333-7, 5333-8, 5333-10, 5334-5, 5334-6, 5334-8, 5334-9, 5334-10, 5334-11, 5335-1. (Stocks were in cold storage until 1989.)] Pl. Aleur. Mutator Stock (Tested positive 1988 47 Mutator activity.) H100/Mu2 (Tested positive for Mutator 1988 47 activity.) Bf1-Mu 044-4 deletion/bm4 (Both Bf1 and 1986 47 bm4 uncovered.) Bf1-Mu (deletion?)/bm4 (Do not uncover 1986 & 1987 47 bm4. Independent isolates - 548-1, 547-6, 544-5, 048-3, 544-9, 546-5, 045-3, 050-8, 046-6, 045-8, 047-7, 549-9, 047-5, 547-5, 548-1, 547-6, 544-6, 544-5, 050-8, 046-6, 045-8, 048-3, 546-5, 547-5, 548-1, 547-6, 544-5, 050-8.) Stand. B70 1988 47 Mutator Stock (Tested positive for Mutator 1988-89 47 activity.) Mutator stock/B733 (Tested positive for 1988 48 ae wx1 1988 48 pr v2 1988-89 48 MO17/Mutator stock (Tested positive for 1988 48 Bf1-Mu/heterozygous TB-9Lc (Bf1-Mu 1986 48 Independent isolates - 547-6, 548-7, 547-6, 544-5, 046-6, 044-7.) Bf1-Mu/heterozygous TB-9Lc (Bf1-Mu 1988 48 Independent isolates - 045-8, 047-7, 048-3, 544-9, 545-2, 046-5, 045-8, 046-1, 047-7, 547-7, 047-7, 048-3, 546-5, 547-5.) Bf1/TB-9Lc (Tested positive for the presence 1988 48 of TB.) [a1-Mum2/a1 sh2 (1:1)] (Crosses segregated 1989 48 for 1, 2 or 3 MuDR's. Stocks used in linkage studies with translocations.) [a1-Mum2 (1:1) Stable kernels X homozygous 1989 48 MuDR]/a1 sh2 (All but one Progeny ear segregated 1:1. One segregated 3;1.) a1 sh2/Intensely mutable a1-Mum2 1989 48 [Stable a1-Mum2 X Stable a1-Mum3 (Yielded 1989 48 progeny with mutable kernels)] (After intercrossing progeny of the original cross for five generations, kernels are intensely mutable.) (Ditto above stock but crossed to a1 sh2.) TB-1Sb/Stand 1989 48 vp5/TB-1Sb 1989 48 +/TB-1Sb-2L 4464 1989 48 TB-3La/Pl. Aleur. wx1 R-Scm2 1989 48 a1 sh2/TB-3La 1989 48 TB-5Sc/Pl. Aleur. wx1 R-Scm2 1989 48 a2/TB-Sc 1989 8 TB-9Sd/Pl. Aleur. wx1 R-Scm2 1989 48 c1 sh1 wx1/TB-9Sb 1989 48 TB-9Lc/Stand. 1989 48 pro1/TB-8Lc 1989 48 TB-10Sc/Stand. 1989 48 y9/TB-10Sc 1989 48 TB-10L19/Pl. Aleur. R-Scm2 1989 49 r/TB-10L19 1989 49 TB-1Sb-2L 4464-4Lf/Pl. Aleur wx1 R-Scm2 1989 49 c2/TB-1Sb-2L 4464-4Lf 1989 49 Stand./homozygous wx1 T7-9 4363 1989 49 Self-pollinated homozygous wx1 T7-9 4363 1989 49 Stand./wx1 wx1 heterozygous T5-9c 1989 49 Self-pollinated wx1 wx1 heterozygous T5-9c 1989 49 Stand./homozygous wx1 T5-9a 1989 49 Self-pollinated homozygous wx1 T5-9a 1989 49 Stand./homozygous wx1 T4-9e 1989 49 Self-pollinated homozygous wx1 T5-9e 1989 49 (wx1-Mum2 reverent/y1 wx1 gl1) (Progeny 1989 49 of self-pollinated ear segregates for Wx1 kernels.) (wx1-Mum5 reverent/y1 wx1 gl1) (Progeny 1989 49 of self-pollinated ear segregates for Wx1 kernels.) [(Bf1-Mu X Bf1)/Stand. Self-pollinated 1989 49 plants did not segregate for Bf seedlings.] Some plants from the selfed ears had Bf tassels and these, when reciprocally crossed to Bf1 Bf1 plants, yielded Bf seedlings. Independent isolates - 044-2, 044-3, 045-1, 045-2, 046-2, 046-9, 047-1, 047-8, 048-6, 048-10, 049-2, 049-4, 049-5, 049-7, 050-4, 050-5, 050-7, 5333-9, 544-4, 544-7, 545-6, 545-7, 048-7. [(Bf1-Mu X Bf1)/Stand. Self-pollinated 1989 50 plants did not segregate for Bf seedlings.] Some plants from the selfed ears did not have Bf tassels, but when reciprocally crossed to Bf1 Bf1 plants, yielded Bf seedlings. Independent isolates: 046-8, 544-3. [(Bf1-Mu X Bf1)/Stand. Self-pollinated 1989 50 plants did not segregate for Bf seedlings.] Some plants from the selfed ears had Bf tassels, but when reciprocally crossed to Bf1 Bf1 plants, yielded no Bf1 seedlings. Independent isolates: 047-4, 047-9, 047-11. [Bf1-Mu X Bf1 X Bf1)/Stand. Self-pollinated 1989 50 plants segregated for only a few Bf seedlings.] Some plants from the selfed ears had Bf tassels and when reciprocally crossed yielded Bf seedlings in both male and female crosses. Independent isolates - 048-4, 049-3, 048-5, 048-8, 048-11, 049-10. (Ditto, continued.) 1989 51 Isolates - 050-9, 546-4, 546-6, 546-8, 548-3, 548-7, 548-8, 550-5, 550-5, 550-7. Homozygous Bf1 1989 51 [yg2-Mu (Del.)/Stand.] (Reciprocal crosses 1989 51 X yg2. Independent isolates - 101-2, 101-8, 103-2, 115-10, 117-3. ISU yg2 1989 51 [a1-Mum2 (1:1) Plants from intensely mutable 1989 51 kernels/Plants from the progeny of a self pollinated Pl. Aleur. Mutator stock, which tested negative for Mutator activity.] Self-pollinated ears segregated for intensely mutable kernels. [a1-Mum2 (1:1) Plants from intensely mutable 1989 51 kernels/Plants from the progeny of a self- pollinated Pl. Aleur. Mutator stock, which tested negative for Mutator activity.] Self pollinated ears segregated for kernels with low or no mutability. Pl. Aleur./[a1-Mum2 (1:1) Plants from 1989 51 intensely mutable kernels/Plants from the progeny of a self-pollinated Pl. Aleur. Mutator stock, which tested negative for Mutator activity.] Self pollinated ears segregated for kernels with low or no mutability. [Pl. Aleur./a1-Mum2 (1:1) intensely mutable 1989 51 kernels] (Self-pollinated ears segregated for mutable kernels.) [Pl. Aleur./Pl. Aleur X a1-Mum2 (1:1) 1989 51 intensely mutable kernels] (Self- pollinated ears segregated for mutable kernels. Outcrosses of above selfs.) [Pl. Aleur./a1-Mum3 (1:1) intensely mutable 1989 51 kernels] (Self-pollinated ears segregated for mutable kernels.) [Pl. Aleur./Pl. Aleur. X a1-Mum3 (1:1) 1989 51 intensely mutable kernels] (Self- pollinated ears segregated for mutable kernels. Outcrosses of above selfs.) [bz1/bz1-Mum3 Stable X MuDR (from a1-Mum2 1989 52 stock)] (1;1) [bz1-Mum9/MuDR (from a1-Mum2 stock)] Self- 1989 52 pollinated, yielded 3:1. [bz1/bz1-Mum9 X MuDR (from a1-Mum2 stock)] 1989 52 (1:1) B73 (Inbred) 1989 52 MO17 (Inbred) 1989 52 Stand/yg2-Mum (Independent isolates - 1, 2, 1989 52 3, 4, 5, 6, 7, 8, 9, 10.) Pl. Aleur./[a1-Mum3 (1:1) intensely mutable] 1989 52 Self-pollinated plants segregated mutable kernels. Pl. Aleur./[a1-Mum3 (1:1) intensely mutable] 1989 52 Self-pollinated plants segregated stable kernels. Stand./ Mu16 (New series of intercrosses.) 1989 52 (Tested positive for Mutator activity.) Mu32 (New series of intercrosses.) (Tested 1989 52 positive for Mutator activity.) (yg2-Mu/yg2) X Stand. (Independent isolates - 1989 52 109-2, 102-5, 113-2.) (In cold storage until 1989-90.) c1 sh1 bz1 wx1 (In cold storage until 1987 52 1989-90.) a1 wx1/(wx1 T2-9a X a1-Mum2) (a1-Mum2 stock 1989-90 52 has one MuDR linked to the break point on chromosome 2 and one unlinked.) a1 wx1/(wx1 T1-9 8389 X a1-Mum3) (a1-Mum3 1989-90 52 stock has one MuDR linked to the chromosome one break point and two unlinked.) a1 wx1/(wx1 T4-9e X a1-Mum2) (a1-Mum2 stock 1989-90 52 has one MuDR linked to the chromosome four break point and two unlinked.) a1 wx1/(wx1 T4-9b X a1-Mum2) (a1-Mum2 stock 1989-90 52 has one MuDR linked to the chromosome four break point and one unlinked.) a1 wx1/(wx1 T4-9b X a1-Mum2) (a1-Mum2 stock 1989-90 52 has one MuDR linked to the chromosome four break point and two unlinked.) (yg2-Mu deletion 102-5/Stand.) X yg2 1989-90 52 (Reciprocally crossed.) (yg2-Mu deletion 113-2/Stand.) X yg2 1989-90 52 (Reciprocally crossed.) [(Bf1-Mu 047-4 X Bf1)/Stand. Self- 1988 52 pollinated - did not segregate for Bf seedlings.] In 1989, some of the progeny of the self had Bf tassels, but when reciprocally crossed to Bf1 plants yielded no Bf seedlings in male or female progeny. Some sibling plants from the same self progeny grown in 1990 with Bf tassels when self-pollinated yielded no Bf seedlings, but when outcrossed to Bf1 yielded progenies that segregated for Bf seedlings. (In cold storage until 1990.) [(Bf1-Mu 047-4 X Bf1)/Stand. Self- 1988 52 pollinated - did not segregate for Bf seedlings, sibling plant to the self- pollinated plant in entry above.] Some plants from the progeny of the self that had Bf tassels were self-pollinated and yielded no Bf seedlings, but when outcrossed to Bf1, yielded a progenies that segregated for Bf seedlings. (In cold storage until 1990.) [(Bf1-Mu 047-1 X Bf1)/Stand.] (Tested in the 1988 52 same manner and yielded the same results as the entry preceding the last one above.) (In cold storage until 1990.) [(Bf1-Mu 544-3 X Bf1)/ Stand. Self- 1988 52 pollinated - did not segregate for Bf seedlings.] In 1989, no plants from the progeny of the self produced Bf tassels, but some yielded Bf seedlings when crossed reciprocally to Bf1 plants in both the male and female outcrosses. In 1990 self-pollinated sibling plant of those planted in 1989 segregated for Bf seedlings. (In cold storage until 1990.) [(Bf1-Mu 044-3 X Bf1)/Stand.] (Ditto) 1988 52 Bf1-Mu 046-1 (Bf seedlings are yellow 1988 52 green or yellow green mutable.) (In cold storage until 1990.) [a1 sh2/a1-Mum2 (1:1)] (In cold storage 1987 52 until 1990.) [a1 sh2/a1-Mum3 (1:1)] (In cold storage 1987 52 until 1990.) [a1 sh2/a1-Mum3 (1:1)] (In cold storage 1988 52 until 1990.) [wx1 T2-9d/a1-Mum2 mutable kernel from (1:1)] 1989 52 (This stock, when crossed with a1 wx1, yielded ears that had one MuDR element linked to the break point in chromosome two and no additional MuDR's or one or two additional unlinked MuDR's.) [wx1 T4-9b/a1-Mum2 mutable kernel from (1:1)] 1989 52 (This stock, when crossed with a1 wx1, yielded ears that had one MuDR element linked to the break point in chromosome four and no additional MuDR's or one or two additional unlinked MuDR's.) [wx1 T4-9e/a1-Mum2 mutable kernel from (1:1)] 1989 52 (This stock, when crossed with a1 wx1, yielded ears that had one MuDR element linked to the break point in chromosome four and one or two additional unlinked MuDR's.) [wx1 T2-9d/a1-Mum3 mutable kernel from (1:1)] 1989 52 (This stock, when crossed with a1 wx1, yielded ears that had one MuDR element linked to the break point in chromosome two and one additional unlinked MuDR.) yg2-Mu (doubtful mutants)/Stand. 1983 52 Independent - isolates: 106-10, 109-2, 110-3, 111-3, 111-6, 112-3, 112-4, 113-4, (Continuation of above) - 114-2, 114-4, 53 116-9, 117-6, 105-1, 105-6, 110-1, 111-1, 112-1,112-5, 113-8, 115-9, 112-8, 113-3, 115-6, 116-3, 116-5, 116-7, [(Bf1-Mu X Bf1)/Stand. Self-pollinated 1988 53 plant, did not segregate for Bf seedlings.] In 1989, some plants from the progeny of the selfs had Bf tassels and when reciprocally crossed to Bf1, yielded progeny that segregated for Bf seedlings in both the male and and female crosses. In 1990, other plants from the progeny of the selfs, with Bf tassels, yielded no Bf seedlings when self-pollinated, but segregated for Bf seedlings when outcrossed to Bf1 plants. The following independent isolates gave these results: 044-2, 046-4, 048-10, 049-2, 050-5, 544-4. (In cold storage until 1990.) [(Bf1-Mu 049-5 X Bf1)/Stand. Self- 1988 53 pollinated plant, did not segregate for Bf seedlings.] In 1989, some plants from the progeny of the self had Bf tassels and when reciprocally crossed to Bf1, yielded progeny that segregated for Bf seedlings in both male and female crosses. In 1990, other plants from the progeny of the self, with Bf tassels, yielded Bf seedlings when self pollinated and in outcrosses to Bf1. (In cold storage until 1990.) (Bf1 X Bf1-Mu 046-1)/Stand. (Bf1-Mu pale 1989 53 green mutable.) [Bf1-Mu (deletion?)/TB-9Lc] X Stand. 1989 53 Independent isolates - 045-8, 048-3, 544-9, 656-5, 547-7. [Bf1-Mu 044-4 (deletion, includes Bf1 & bm4) 1989 53 X bm4 (bm plant)]/Stand. [(Bf1-Mu X Bf1)/Stand. Self-pollinated, did 1989 53 not segregate for Bf seedlings.] Some of the plants of the progeny of the self had Bf tassels that when reciprocally crossed to Bf1, segregated Bf seedlings in progeny of both male and female crosses. The following independent isolates gave the above results: 5327-2, 5327-6, 5328-3. (Pl. aleur. wx1 X a1 wx1)/a1 wx1 1988-89 53 [yg2-Mu (deletion) X Stand.]/yg2 (Independent 1984 53 isolates - 104-7, 107-1, 108-3, 116-10, 117-5, 107-2.) Bf1 (Homozygous) 1989 53 [(Bf1-Mu X Bf1)/Stand. Self-pollinated, did 1989 53 not segregate for Bf seedlings.] (Independent isolates - 5328-6, 5328-7, 5329-2, 5329-3, 5329-9, 5230-1, 5330-5, 5331-2, 5331-7, 5332-1, 5319-3, 5333-5, 5333-7, 5334-1, 5334-5, 5334-8, 5334-11.) vp5-Mum1/TB-1Sb 1988 53 lw1/TB-1La 1988 53 al-Brawn/TB-2Sb 1988 53 w3/TB-1Sb-2L 4464 1989 54 cl1 cl1 Clm3 Clm3/TB-3Sb 1989 54 a1 sh2/TB-3La 1989 54 su1 gl4/TB4-4Sa 1988 54 c2/TB-4Lf 1989 54 a2 or a2 bm1 bt1/TB-5Sc 1989 54 lw2/TB-5La 1988 54 dek28/TB-6Sa 1988-89 54 y1 wx1 gl1/TB-6Lc 1988 54 vp9/TB-7Sc 1989-90 54 o5/TB-7Lb 1988 54 pro1/TB-8Lc 1989 54 c1 sh1 wx1/TB-9Sd 1989 54 TB-9Lc 1988 54 (y9/+)/Tb-10Sc 1989 54 r1/TB-10L19 1989 54 [(Bf-Mu X Bf1/Stand. Self-pollinated, Did not 1990 54 segregate Bf seedlings.] In 1989, some plants from the progenies of the selfs had Bf tassels, which when reciprocally crossed to Bf1 segregated for Bf seedlings in the male and female outcrosses. In 1990, some plants from progenies of selfs of sibling plants to the same (Bf1-Mu X Bf1/Stand.). and those from the progeny of the original self, with Bf tassels, were self- pollinated and outcrossed to Bf1. The selfed plants did not segregate for Bf seedlings while the outcrosses did. Independent isolates - 044-2, 046-2, 048-10, 049-2, 544-6, 050-5, 544-4, 049-5. Bf1-Mu 046-1 (yellow green or pale green 1990 54 mutable)/Bf1 (Reciprocal crosses.) [(Bf1-Mu X TB-9Lc) bf bm plant]/Bf1 1990 54 (reciprocal crosses.) [(Bf1-Mu 044-4, deletion X bm4) bm plants/ 1990 54 Stand.] X Bf1 (Reciprocal crosses.) (Ditto) 1990 55 [Bf1-Mu 046-6 (deletion) X bm4]/Stand. 1990 55 (Reciprocal crosses.) [(Bf1-Mu 044-3 X Bf1/Stand. Self- 1990 55 pollinated, did not segregate for Bf seedlings.] In 1989, no plants in the progeny of the self had Bf tassels but some, when reciprocally crossed to Bf1, yielded Bf seedlings in both male and female crosses. In 1990, some sibling plants from the progeny of the self where again self-pollinated and segregated for Bf seedlings. [(Bf1-Mu X Bf1)/Stand. Self-pollinated, 1990 55 segregated for no Bf seedlings.] In 1989, some plants in the progeny of the selfs had Bf tassels, but did not segregate for Bf seedlings in either the male or female progeny when reciprocally crossed to Bf1. In 1990, sibling plants from the progeny of the selfs, with Bf tassels, where self-pollinated and some yielded progeny that segregated for Bf seedlings and some did not segregate for Bf Seed- ings. Independent isolates - 047-7, 047-9, 047-1. [(Bf1-Mu X Bf1)/Stand. Self-pollinated, 1990 55 segregated for no Bf Seedlings.] In 1989, some plants from the progeny of the selfs had Bf tassels and when these were reciprocally crossed to Bf1, the progeny in both the male and female crosses segregated for Bf seedlings. In 1990, sibling plants from the progeny of the selfs, with Bf tassels, when self-pollinated and outcrossed to Bf1, yielded Bf seedlings in both the self and outcross progeny. Independent isolates - 5319-3, 5333-5, 5333-7, 5334-5, 5334-8, 5334-11. Bf1 1990 55 [yg2-Mu (deletion)/Stand.] (Reciprocally 1990 55 crossed to yg2. Male crosses had a low frequency of yg seedlings.) Independent isolates - 106-10, 110-2, 110-3, 111-6, 113-4. wd-Mu (Independent isolates - 1, 2, 3, 4, 5. 1988 55 Self-pollinated and crossed to yg2.) [(Bf1-Mu X Bf1)/Stand. Self-pollinated, 1990 56 did not segregate for Bf seedlings.] In 1989, some plants from the progeny of the selfs had Bf tassels that when reciprocally crossed to Bf1 plants did not segregate for Bf seedlings in both the male and female outcrosses. In 1990, sibling plants from the self progeny with Bf tassels were self- pollinated and outcrossed to Bf1 plants. No Bf seedlings were observed in the progeny of the selfs but some of the outcross progenies segregated for Bf seedlings. Independent isolates - 047-4, 047-9, 047-11. [(Bf1-Mu 544-3 X Bf1)/Stand. Self-pollinated, 1990 56 did not segregate for plants with Bf seedlings.] None of the plants from the progeny of the self had Bf tassels, but some plants segregated for Bf seedlings when selfed and outcrossed to Bf1. Progeny of the second self selfed again and outcrossed to Stand. and Bf1. [(Bf1-Mu 044-3 X Bf1)/Stand. Self-pollinated, 1990 57 did not segregate for plants with Bf seedlings.] The rest of the description is the same as the preceding entry. Bf1 bm4 (Stock segregating these mutants.) 1990 57 Intercrosses between different [(Bf1-Mu X 1990 57 Bf1)/Stand. Self-pollinated, did not segregate for Bf seedlings.] In 1989, plants from the progeny of the of the selfs with Bf tassels were reciprocally crossed to Bf1. No Bf seedlings were observed in either the male or female outcross progeny. In 1990, intercrosses involved sibling plants, with Bf tassels, from different Bf1-Mu isolates were made and the parent plants used in the intercrosses were outcrossed to Bf1. In a few instances, one of the parent plants used to make an intercross was self-pollinated. All progenies from the intercrosses segregated for Bf seedlings as did the progenies of the outcrosses to Bf1. Most of the progenies from the self- pollinated plants did not segregate for Bf seedlings but several did. a1 sh2 1990 57 a1 wx1/[wx1 T2-9d X a1-Mum2 mutable from 1990 57 (1:1)] (One MuDr linked to the break point in chromosome two.) a1 wx1/[wx1 T2-9d X a1-Mum2 mutable from 1990 57 (1:1)] (One MuDR linked to the break point in chromosome two and one independent MuDR.) a1 wx1/[wx1 T2-9d X a1-Mum2 mutable from 1990 57 (1:1)] (One MuDR linked to the break point in chromosome two and two independent MuDR's.) a1 wx1/[wx1 T4-9b X a1-Mum2 mutable from 1990 57 (1:1)] (One MuDR linked to the break point in chromosome four and two independent MuDR's.) a1 wx1/[wx1 T4-9e X a1-Mum2 mutable from 1990 57 (1:1)] (One MuDR linked to the break point in chromosome four and two independent MuDR's.) a1 wx1/[wx1 T2-9d X a1-Mum3 mutable from 1990 57 (1:1)] (One MuDR linked to the break point in chromosome two.) a1 wx1 1990 57 Stand. Q60 (Q66/Q67) 1990 57 Stand. B70 (B77/B79) 1990 57 a1 wx1 1990 58 a1 sh2 1990 58 [(yg2-Mu (deletion) X yg2] yg plant/Stand. 1990 58 Isolates: 104-7, 107-1, 108-3, 116-10, 117-5, 107-2,106-3. ISU yg2 1989 58 wd-Mu X (wd1/+) (wd-Mu's - 1, 2 3, 4, 5, 6.) 1991 58 (wd1/+) (Self-pollinated) 1991 58 wd-Mu X (pyd/+) (wd-Mu's - 1, 2, 3, 4, 5, 1991 59 6, 7, 8, 9, 10, 11.) (pyd (Mc Clinock's stock)/+) (Self- 1991 59 pollinated) ISU yg2 1991 59 (wd-Mu6/+) X yg2 1991 59 (yg2-Mu 120-6/+) X yg2 1991 59 (v28/+) (Self-pollinated) 1991 59 Stand. X (wd1/+) 1991 59 Pl. Aleur. X [pyd (Mc Clintock's stock)/+] 1991 59 Stand. X [pyd (Mc Clintock's stock)+] 1991 59 Stand./(v28/+) 1991 59 (wd-Mu/+)/yg2 (Independent isolates - 1, 2, 1991 60 3, 4, 5) (Stand./wd-Mu6) (Self pollinated) (In cold 1986 60 until 1991.) (wd-Mu7 X Stand.)/yg2 (In cold storage 1984 60 until 1991.) (wd-Mu8 X Stand.)/yg2 (In cold storage 1984 60 until 1991.) (wd-Mu9 X Stand.)/yg2 (In cold storage 1984 60 until 1991.) (wd-Mu10 X Stand./yg2 (In cold storage 1984 60 until 1991.) (wd-Mu11 X Stand./yg2 (In cold storage 1984 60 until 1991.) (Stand./wd-Mu6) (Self-pollinated) (In cold 1986 60 storage until 1991.) [yg2-Mu 106 (deletion)] X yg2]/C1 sh1 wx1 1989 60
